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Table 2 Retention time and estrogenic response of indicative compounds

From: Assessment of xenoestrogenic exposure by a biomarker approach: application of the E-Screen bioassay to determine estrogenic response of serum extracts

Compound

Rt

MOEC

RPP

  

μM

RPE ± SD

 

Endogenous compounds

Cholesterol

1.83

0.0001–10

Nonactive

-

5α-dihydroprogesterone

4.14

10

58 ± 3

0.01

Progesterone

5.03

25

16 ± 2

0.004

Pregnenolone

6.07

10

92 ± 6

0.01

Androsterone

6.21

10

97 ± 3

0.01

5α-dihydrotestosterone

6.63

1

64 ± 5

0.1

Androstenedione

6.80

10

16 ± 2

0.01

Dehydroepiandrosterone

6.86

1

92 ± 2

0.1

Testosterone

8.23

10

34 ± 5

0.01

Estrone

9.06

0.01

84 ± 8

10

17α-hydroxyprogesterone

9.23

10

7 ± 1

0.01

17α-hydroxypregnenolone

12.78

10

30 ± 1

0.01

17β-estradiol

14.83

0.001

100 ± 7

100

16α-hydroxyestrone

22.86

0.01

77 ± 2

10

Estriol

24.01

0.01

103 ± 3

10

Organochlorines

Hexachlorobenzene

1.34

0.001 – 50

Nonactive

-

PCB180

1.40

0.001 – 50

Nonactive

-

p,p'-DDE

1.43

12.5

92 ± 2

0.008

PCB153

1.48

0.001 – 50

Nonactive

-

PCB138

1.49

0.001 – 50

Nonactive

-

o,p'-DDT

1.53

6.25

96 ± 7

0.016

Dieldrin

1.75

25

44 ± 9

0.004

Methoxychlor

2.29 + 2.88

12.5

57 ± 8

0.008

4-OH-CB146

2.67

0.001 – 50

Nonactive

-

4-OH-CB187

2.67

50

24 ± 4

0.002

4-OH-CB107

2.70

0.001 – 50

Nonactive

-

β-hexachlorocyclohexane

4.13

2.5

61 ± 2

0.04

  1. Abbreviations: Rt, retention time; MOEC, maximum observed effect concentration; RPE, relative proliferative effect; RPP, relative proliferative potency