Figure 1

Observation of N2a-Rα cells by phase contrast microscopy. The parental Neuro2a cells (panel "Neuro2a") were transfected with rat AhR in the mammalian expression vector pcDNA4/V5-His and selected with zeocin. The morphology of the resultant transfectant is shown in panel "N2a-Rα ". The cells transfected with the vector without the insert are shown in panel "N2a-Vc". The N2a-Rα cells exhibited spontaneous neurite outgrowth. Note that no morphological differences in N2a-Vc were observed compared with the parental Neuro2a cells.